| 학회 |
한국고분자학회 |
| 학술대회 |
2014년 가을 (10/06 ~ 10/08, 제주 ICC) |
| 권호 |
39권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Effect of graphene and graphene oxide on tonsil –derived stem cells in polypeptide thermogel |
| 초록 |
The poly(ethylene glycol)-poly(L-alanine) diblock copolymer undergoing sol-to gel transition when the temperature increased was synthesized by ring opening polymerization, and was applied to 3D cell culture of human tonsil-derived mesenchymal stem cells(TMSC). The TMSC were encapsulated in the thermogel with graphene and graphene oxide. Cell proliferation and viability were assayed by CCK-8 and Live/Dead methods. The gene and protein expression levels in the cells were analyzed by using real time qPCR and immunofluorescence staining. The result of this study shows that TMSC differentiation in 3D environments depends on graphene and graphene oxide concentration. |
| 저자 |
박진혜, 고두영, 남상아, 정희정, 정병문
|
| 소속 |
이화여자대 |
| 키워드 |
hydrogel; 3d cell culture; stem cell; graphene; graphene oxide
|
| E-Mail |
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