| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Engineering Soluble PD-1 for Improved Anti-Cancer Therapy |
| 초록 |
Although therapeutic antibodies, which inhibit the interaction between PD-1 (Programmed cell death protein 1) and PD-L1 (Programmed cell death-ligand 1), have revolutionized in the field of immuno-oncology, they have a significant limitation with poor tumor penetration. To address the issue, we engineered the ectodomain of PD-1 using bacterial display and flow cytometric high-throughput library screening, and a variety of novel PD-1 variants exhibiting improved binding affinity to PD-L1 were successfully isolated. After production of the resulting novel PD-1 variants in mammalian cells, their various physicochemical and biological functions, including T cell activation, were thoroughly characterized. The isolated PD-1 variants will provide a promising platform for various pharmaceutics for PD-1 / PD-L1 blockade. |
| 저자 |
하지연1, 천광진2, 고상환1, 이호원3, 황옥경3, 하경수3, 고병준4, 정상택1
|
| 소속 |
1고려대, 2뉴라클사이언스, 3오송첨단의료산업진흥재단, 4성신여자대 |
| 키워드 |
PD-1; PD-L1; Immune checkpoint; Immunotherapy; Protein engineering
|
| E-Mail |
|
