| 초록 |
We developed non-covalently PEGylated ternary-complex of siRNA using poly(2-dimethylamino)ethyl methacrylate [PDMAEMA] and its copolymer with poly(a-methylether-u-methacrylate-ethyleneglycol) [PMAPEG]. After orotracheal administration to fibrotic Sprague Dawley (SD) model rats, FAM-labeled siCTGF complex was effectively delivered to the cells in the lung. The siCTGF/PDMAEMA/PDMAEMA-b-PMAPEG complex resulted in a significant reduction in target gene expression, collagen deposition, inflammatory cytokines production, and drastic attenuation of pulmonary fibrosis in pathophysiological analysis. Furthermore, the survival rate was remarkably increased to the statistically significant level in comparison with the scrambled siCTGF treatment group. |
