| 학회 |
한국화학공학회 |
| 학술대회 |
2012년 봄 (04/25 ~ 04/27, 제주 ICC) |
| 권호 |
18권 1호, p.432 |
| 발표분야 |
생물화공 |
| 제목 |
Honeys Reduce Biofilm Formation and Virulence in Pathogenic Escherichia coli |
| 초록 |
Honey at 0.5% (v/v) significantly reduced biofilm formation in enterohemorrhagic Escherichia coli O157:H7 without affecting the growth of planktonic cells, while it did not harm commensal E. coli K-12 biofilm. Transcriptome analyses show that honey significantly repressed curli genes (csgBAC), quorum sensing genes (AI-2 importer and indole biosynthesis), and virulence genes (LEE genes). Glucose and fructose in the honeys were found to be key components in reducing the biofilm formation of E. coli O157:H7 through the suppression of curli production and AI-2 importer. Furthermore, honey decreased the colonization of E. coli O157:H7 cells on human HT-29 epithelial cells. In contrast, glucose had a temperature-dependent effect on commensal E. coli K-12 biofilm formation. These results suggest that low concentrations of honey, such as in honeyed water, can be a practical means for reducing the colonization and virulence of pathogenic E. coli O157:H7 without harming commensal E. coli community. |
| 저자 |
이진형1, 박주현1, 김정애1, Ganesh Prasad Neupane1, 조무환1, 이창수2, 이진태1
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| 소속 |
1영남대, 2충남대 |
| 키워드 |
Honey; E. coli; Biofilm; Curli; Quorum Sensing; Virulence; Glucose
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| E-Mail |
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| 원문파일 |
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