| 학회 |
한국화학공학회 |
| 학술대회 |
2018년 가을 (10/24 ~ 10/26, 대구 EXCO) |
| 권호 |
24권 2호, p.1497 |
| 발표분야 |
생물화공(Biochemical Engineering) |
| 제목 |
Optimization of high cell density culture condition using Methylomonas sp. DH-1 by chemostat |
| 초록 |
Methylomonas sp. DH-1 is a type I methanotroph that uses methane as a carbon source. This bacterium is one of the brightest and most promising strains near future in that it can use methane gas, one of the harmful green-house gases that cause global warming. In this study, Methylomonas sp. DH-1 was cultured in continuous fermenter for high cell density culture by optimizing culture medium and physical condition. Prior to chemostat culture, DH-1 cells were pre-cultured until proper cell optical density value that means cell density. Then we started chemostat culture using peristaltic pump by dilution rate 0.05/hour. Through chemostat, we changed culture medium, agitation rate, and gas flow rate and judged that equilibrium reached when cell optical density remained constant for a certain time. Brief summary of the experimental results is as follows. Cell activity was higher in AMS medium than in NMS medium, and the higher gas flow rate, the higher optical density value was measured. Similarly, the higher agitation rate, the higher optical density was measured. Agitation rate has more impact on cell growth compared with gas flow rate. |
| 저자 |
김성일1, 한준희1, 김현수1, 조숙형2, 나정걸1, 이진원1
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| 소속 |
1서강대, 2C1가스 리파이너리 사업단 |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
초록 보기 |
