| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Optimization of flux distribution between the glyoxylate and TCA cycle for efficient tyrosine production from acetate in Escherichia coli |
| 초록 |
Acetate is one of promising feedstocks owing to its cheap price and great abundance. Here, we engineered a previously reported strain, SCK1, for efficient production of tyrosine from acetate. Initially, the acetate uptake and gluconeogenic pathway were amplified to maximize the flux toward tyrosine. As flux distribution between glyoxylate and TCA cycles is critical for efficient precursor supplementation, the activity of the glyoxylate cycle was precisely controlled by expression of isocitrate lyase gene under different-strength promoters. Consequently, the engineered strain with optimal flux distribution produced 0.70 g/L tyrosine with 20% of the theoretical maximum yield which are 1.6-fold and 1.8-fold increased values of the parental strain. This is the first demonstration of tyrosine production from acetate. Our strategies would be widely applicable to the production of various chemicals from acetate in future. |
| 저자 |
민명원1, 조민지1, 노명현1, 임현규2, 강채원1, 임대균3, 오민규3, 정규열1
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| 소속 |
1포항공과대, 2UCSD, 3고려대 |
| 키워드 |
precise flux redistribution; tyrosine; acetate
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| E-Mail |
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