| 학회 |
한국공업화학회 |
| 학술대회 |
2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center)) |
| 권호 |
24권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Electrical stimulation facilitates differentiation of chemically induced cardiac spheroids |
| 초록 |
Direct reprogramming technique which converts somatic cells to other lineage cells has been attracting attention because this method can easily generate the desired cell types without using stem cells. However, there is still a need for improvement in reprogramming efficiency. Here, we generated 3D cardiac spheroids using direct reprogramming with small molecules. We confirmed that cardiac-specific markers were expressed in formed 3D spheroids and gene expression levels associated with cardiac differentiation were upregulated compared to 2D direct reprogramming. To further enhance reprogramming efficiency, we briefly screened the voltages which can facilitate 3D cardiac reprogramming and affect the beating of induced cardiac cells. Electrically stimulated spheroids showed upregulated cardiac gene expression compared with the ones without stimulation. This research was supported by a grant (19172mfds168) from Ministry of Food and Drug Safety in 2020. |
| 저자 |
민성진, 진윤희, 김유흔, 정은선, 김수겸, 김수연, 조승우
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| 소속 |
연세대 |
| 키워드 |
electrical stimulation; direct reprogramming; cardiac spheroids
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| E-Mail |
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