| 학회 |
한국화학공학회 |
| 학술대회 |
2012년 가을 (10/24 ~ 10/26, 부산 BEXCO) |
| 권호 |
18권 2호, p.1875 |
| 발표분야 |
생물화공 |
| 제목 |
Cloning and expression of a xylanase gene from newly isolated Staphylococcus sp. SS8 |
| 초록 |
From 11 strains, which is isolated from various environmental samples, SS8 microbial strain was characterized and identified as Staphylococcus sp. by analysis of 16S rDNA sequence and biochemical studies, and named as Staphylococcus sp. SS8 which has high xylanase activities. The optimum temperature and pH for xylanase activity of Staphylococcus sp. SS8 were 50℃ and 9.0, respectively. The xylanase activity was strongly inhibited by Al+++. The xylanase gene was cloned from Staphylococcus sp. SS8 genomic DNA by polymerase chain reaction (PCR). The amplified PCR product was ligated with the T&A cloning vector system and the constructed plasmids were transformed into E. coli DH5α. The sequence analysis of the insert DNAs revealed the identification of a 640-bp region containing xylanase open reading frame. According to xylanase gene sequence analysis, Staphylococcus sp. SS8 had gene sequence similarity of 99% with Bacillus subtilis Xyl gene for xylanase (AB457186.1). |
| 저자 |
김중곤, 박유리, 차영록, 안기홍, 윤영미, 박선태, 문윤호, 안종웅, 구본철, 박광근
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| 소속 |
농촌진흥청 국립식량과학원 바이오에너지 작물센터 |
| 키워드 |
xylanase; Staphylococcus sp.; cloning
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| E-Mail |
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| 원문파일 |
초록 보기 |
