| 학회 |
한국화학공학회 |
| 학술대회 |
2019년 가을 (10/23 ~ 10/25, 대전컨벤션센터) |
| 권호 |
25권 2호, p.1509 |
| 발표분야 |
생물화공 (Biochemical Engineering) |
| 제목 |
Electrode mediated energy recovery and acetate oxidation by P.putida 2523 in a microbial fuel cell |
| 초록 |
Pseudomonas putida is recently highlighted for versatile carbon metabolism and also known as electro-active in a microbial fuel cell (MFC). Hence, P. putida is an ideal host strain for electrode-based metabolic regulation to produce platform chemicals. In this study, we examined the effect of electrode anaerobic growth of P. putida in different culture condition such as conventional fermentation and anode respiring environment (closed circuit MFC) using acetate as carbon source. The cell growth of P. putida was maintained in the presence of the electrode with simultaneous electricity generation. This strain has discharged electrons up to 110µA/cm2 of anodic current. Further acetyl-CoA synthetase activity assay and regulated NADH/NAD+ ratio support that P. putida can respire to an electrode and oxidize acetate. These results imply that the carbon electrode in MFC may improve utilization of refractory substrate such as acetate. Further metabolic engineering and optimization of P.putida may provide a platform for bioenergy production and BES based bio-refinery process. |
| 저자 |
Sakuntala Mutyala, 송영은, 백지윤, 설은희, 김중래
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| 소속 |
부산대 |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
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