| 학회 | 한국화학공학회 |
| 학술대회 | 2005년 가을 (10/21 ~ 10/22, 인하대학교) |
| 권호 | 11권 2호, p.2019 |
| 발표분야 | 생물화공 |
| 제목 | Optimization of fed-batch culture strategy for the production of recombinant antibody by rCHO cells |
| 초록 | Fed-batch culture of mammalian cell has been widely utilized for production of therapeutic proteins. Major advantages of fed-batch culture over batch and perfusion cultures were increase of maximal viable cell concentration and prolongation of culture lifetime for a high product concentration. Conventional strategies for fed-batch culture were based upon replenishment of consumed nutrients by feeding supplemental medium and reduction of ammonia and lactate accumulation by control of glucose and glutamine concentration. Various research groups have developed and optimized their own feeding strategy for each cell line and target product. In this study, basal serum-free medium and supplemental medium for fed-batch culture were developed and they were modified with various statistical methods. Also, nutrient feeding strategies including separate supplementation of each nutrient group and systematic consideration of specific nutrient consumption rate were evaluated for high-yielding production of recombinant antibody. |
| 저자 | 김도윤1, 김병진1, 장호남2, 오덕재3 |
| 소속 | 1한국과학기술원 / 세종대, 2한국과학기술원, 3세종대 |
| 키워드 | fed-batch culture; recombinant antibody; Chinese Hamster Ovary cell; serum-free medium |
| 원문파일 | 초록 보기 |
