| 학회 | 한국화학공학회 |
| 학술대회 | 2005년 가을 (10/21 ~ 10/22, 인하대학교) |
| 권호 | 11권 2호, p.2054 |
| 발표분야 | 생물화공 |
| 제목 | Temperature, a simple but a key environmental parameter that influences cell growth and antibody production the culture of chinese hamster ovary cells |
| 초록 | In animal cell culture, temperature is one of the key parameters which influence cell growth and recombinant protein production. Generally, most of mammalian cell cultures are performed at 37℃ which is normal human body temperature. In this study, to evaluate the effect of temperature on recombinant antibody production, rCHO cells were cultivated at various culture temperatures (33℃, 37℃ and 39℃). When the temperatures were lower than 37℃, cell growth was suppressed, but cell viability maintained at for longer culture periods. When the temperature was lowered from 37℃ to 33℃, the specific productivity was three times higher compared to the control. In contrast, when the temperature was heighten from 37℃ to 39℃, the specific growth rate was increased. Interestingly, when temperature was shifted instantaneously from 37℃ to 4℃ for 5 minutes, significant of increase glucose consumption was observed, which reveals dramatic changes in cellular metabolic activities driven by cold culture environments. |
| 저자 | 김수연, 설삼숙, 오덕재 |
| 소속 | 세종대 |
| 키워드 | CHO cell; culture temperature; recombinant antibody |
| 원문파일 | 초록 보기 |
