| 초록 |
Coculturing stem cells with the desired cell type is an effective method to promote the differentiation of stem cells. The features of membrane used for coculture are crucial to achieving the best outcome. Not only should the membrane act as a physical barrier that prevents the mixing of the cocultured cell populations, but it should also allow effective interactions between the cells. Unfortunately, conventional membranes used for coculturing do not sufficiently meet the requirements. Also, cell harvesting using proteolytic enzymes following coculture impairs the cell viability and extracellular matrix produced by the cultured cells. To overcome these problems, we developed a PNIPAAM–functionalized, nano-thin, and, highly porous (NTHP) membrane for the generation of transfer-printable, differentiated cell sheets derived from stem cells through coculture. The developed coculture platform may be effective for producing therapeutic multilayered sheets of various types of cells. |
