| 학회 |
한국화학공학회 |
| 학술대회 |
2016년 봄 (04/27 ~ 04/29, 부산 BEXCO) |
| 권호 |
22권 1호, p.522 |
| 발표분야 |
생물화공 |
| 제목 |
Development of Specific HbA1c-binding DNA Aptamers for Point-of-care Test of Diabetes mellitus |
| 초록 |
Diabetes mellitus has become a major health problem worldwide, and the proportion of people who suffer from diabetes is increasing. Thus, the early diagnosis has more important for diabetes patients. Hemoglobin A1c (HbA1c) is a significant marker protein suitable for long-term monitoring of glycemic control. Aptamers are single-stranded DNA or RNA that can serve as antibody mimics because it has advantages of high affinity for their target molecules and high stability. In this study, ssDNA aptamers were screened by SELEX process. We demonstrated specific binding affinity of aptamers with HbA1c using real-time PCR. We obtained Kd value of aptamer candidates for selection of best aptamer using SPR assay. We performed competition test between selected aptamers and antibody. These aptamers proved its potential use in rapid detection of HbA1c via point-of-care diagnostic kit. This study was supported by Fund of Biomedical Research Institute, Chonbuk National University Hospital (20120700). |
| 저자 |
김서경1, 이상희1, 박태선2, Simranjeet Singh Sekhon1, 민지호2, 김양훈1
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| 소속 |
1충북대, 2전북대 |
| 키워드 |
의약생물공학 |
| E-Mail |
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| 원문파일 |
초록 보기 |
