| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 |
19권 1호 |
| 발표분야 |
우수논문(구두) |
| 제목 |
Graphene Oxide-Peptide Hybrids for Optical Detection of Matrix Metalloproteinase-2 by Fluorescence Turn-on Response |
| 초록 |
Graphene oxide (GO) is a novel material that has been rapidly growing in the field of material science with its remarkable physicochemical properties. Especially, photoluminescence of GO suggest that it has great potential for optical sensing area with photo-stability and unique photoluminescence (PL) property appearing in the visible and near-infrared region. In addition, GO based sensing platform have attracted great attentions due to the featured properties of GO, such as biocompatibility, and chemical affinity to biomolecules. Herein, we present GO PL-based optical biosensor consisting of a luminescent GO donor covalently linked to a peptide-quencher complex for the simple, rapid and sensitive detection of specific proteases. The designed GO-peptide hybrid was able to detect specific proteases, chymotrypsin and matrix metalloproteinase-2 (MMP-2), by the “turn-on” response of quenched GO fluorescence after proteolytic cleavage and releasing the quencher. Furthermore, the GO-peptide-quencher hybrid successfully monitored MMP-2 secreted from living cells with high sensitivity. |
| 저자 |
양진경1, 곽선영2, 전수지2, 김혜인2, 이은진1, 김종호2, 이윤식1
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| 소속 |
1서울대, 2한양대 |
| 키워드 |
Graphene oxide; FRET; Peptide conjugate; MMP-2 detection
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| E-Mail |
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