| 초록 |
CK2α is a serine/threonine kinase that is constitutively active in multiple cancer cells. Using colon cancer cell lines such as HT29 and SW620, CK2α induced epithelial mesenchymal transition and the transitioned cells became more proliferative. We assumed that CK2α could affect cancer cell growth by modulating energy metabolism. Therefore, we examined the reprogrammed metabolic fluxes of CK2α on glucose metabolism via isotope labeled substrates and could successfully determine the relative amount and mass isotopomer distribution(MID) of metabolites using GC/MS. The results showed that continuous activation of CK2α facilitates the Warburg effect and anaplerotic pathway. Besides, results also showed CK2α overexpression affected glutamine uptake metabolism, varying amount and MID of TCA intermediates. This study suggests that because oncogenes regulate important metabolic enzymes and metabolism signaling pathways, CK2α is an excellent metabolic target for cancer therapy. |
