| 초록 |
Functionalization of Qβ virus-like nanoparticles (VLPs) by encapsulation and surface modification technique expands the design possibility of these as building blocks for material science and medicinal applications. Qβ VLPs encapsulating multiple copies of fluorescent proteins were generated using a modular system. The encapsulated proteins were nearly identical in photochemical properties to monomeric analogues, were more stable toward thermal degradation, and were protected from proteolytic cleavage. Residues on the outer capsid surface were chemically derivatized by acylation and azide-alkyne cycloaddition without affecting the fluorescence properties of the packaged proteins. A high affinity carbohydrate-based ligand of the CD22 receptor was thereby attached, and specific cell labeling by the particles was successfully detected and quantified. These VLP system motivate us to develop a cell-targeting photodynamic therapy (PDT) reagent. |
