| 초록 |
One of the most powerful genome editing tool is clustered regularly interspaced short palindromic repeat (CRISPR) associated system (Cas9) with sgRNA. In many studies, Cas9 system is performed by viral vectors, due to high in vivo transfection efficiency despite viral vectors have been reported in connection with clinical trials. Therefore, non-viral delivery of the Cas9 system is more appropriate with clinical trials. In this study, we have directly conjugated 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) to Cas9 protein, which is one of widely used cationic phospholipid to improve delivery efficiency with better safety concerns. We targeted the EGFR single mutation in cancer to determine the genome editing efficiency. We hope that our approach would provide effective delivery of Cas9 system for genome editing and open up new opportunities for clinical trials. |
