초록 |
Carbon monoxide (CO), a major component of industrial waste gas, can be converted into valuable chemicals such as acetate and ethanol. Biological conversion of CO is conducted by specific bacteria harboring the Wood-ljungdahl Pathway (WLP) and CO-dehydrogenase. However the CO conversion rate of the most microorganisms is not sufficient to support commercialized process. We hypothesized that iron oxidation can be coupled with CO uptake via WLP. The iron oxidation supplement the limited reducing equivalent of WLP, thus improve metabolite production from CO conversion. In this study, we attempted to enrich river sediment in the media containing iron bead (Fe0) as external electron source under CO condition, and isolate and characterize the strains adapted. The most of the strains isolated, were characterized as Clostridium species. CO conversion efficiencies of all 9 strains were determined lower than the mixed culture, however NR-1 strain have comparable activity with mixed community. |