| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 가을 (10/11 ~ 10/12, 일산킨텍스) |
| 권호 |
32권 2호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Characterization and preparation of low molecular weight water soluble chitosan nanoparticle modified with cell targeting ligand for efficient gene delivery |
| 초록 |
Gene therapy using LMWSC (low molecular water soluble chitosan) as polycationic polymer shows good biocompatibility, but low transfection efficiency. The mechanism of folic acid (FA) uptake in the cells to promote targeting and internalization could improve transfection rates. The objective of this study was to synthesize and characterize WSCFA-DNA complex and evaluate their cytotoxicity in vitro. In 1H NMR spectra, specific peaks appeared both of Folic acid and LMWSC in D2O. WSCFA nanoparticles have spherical shapes with particle size show below 110nm. In the cell cytotoxicity test, WSCFA-DNA complex showed high cell viability, in vitro. Gel electrophoresis showed condensed DNA within the carriers. In vitro transfection efficiency was assayed by fluorescence spectroscopy. WSCFA nanoparticles have less cytotoxicity, good DNA condensation and particle size around 110nm, which makes them a promising candidate as a non-viral gene vector. |
| 저자 |
허선행, 장민자, 김동곤, 정영일, 장미경, 나재운
|
| 소속 |
순천대 |
| 키워드 |
LMWSC; Folic acid; Nanoparticle; Gene therapy
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| E-Mail |
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