| 학회 |
한국화학공학회 |
| 학술대회 |
2021년 봄 (04/21 ~ 04/23, 부산 BEXCO) |
| 권호 |
27권 1호, p.507 |
| 발표분야 |
생물화공 |
| 제목 |
Novel coenzyme B12 riboswitch-based sensor combined with transcriptional repressor for signal inverting and amplifying |
| 초록 |
Genetic circuits are composed of input, logic, and output parts. Construction of complex circuits for practical applications requires numerous tunable genetic parts. However, the limited diversity and complicated tuning methods used for the input parts hinders the scalability of genetic circuits. Therefore, a new type of input part is required that responds to diverse signals and enables easy tuning. Here, we developed RNA protein hybrid input parts that combine a riboswitch and orthogonal transcriptional repressors. The hybrid inputs successfully regulated the transcription of an output in response to the input signal detected by the riboswitch and resulted in signal inversion because of the expression of transcriptional repressors. Furthermore, the hybrid input detected both exogenous and endogenous signals, indicating potential applications in metabolite sensing. This hybrid in put part could be highly extensible considering the rich variety of components. |
| 저자 |
황윤희1, 정규열1, 장성연2, 장성호3, 노명현1, 임현규4
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| 소속 |
1POSTECH, 2Cornell medicine, 3Incheon Univ., 4UCSD |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
초록 보기 |
