| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 봄 (04/23 ~ 04/25, 제주ICC) |
| 권호 |
14권 1호, p.329 |
| 발표분야 |
분리기술 |
| 제목 |
Simultaneous determination of matrine and oxymatrine in Sophora Flavescens Ait. by high performance liquid chromatography |
| 초록 |
Following a detailed study, a reversed-phase high performance liquid chromatographic method (HPLC) has been developed and validated for analysis of two bioactive alkaloids, matrine and oxymatrine, in Sophora flavescens Ait.. HPLC separation of the alkaloids was performed on a C18 column and detected by ultraviolet absorbance at 210 nm. A mobile phase composed of 0.01 mol/L KH2PO4 buffer–methanol–triethylamine in the ratios 65:35:0.01 (v/v) was found to be the most suitable for this separation at a flow-rate of 0.5 mL/min and enabled the baseline separation of the two analytes free from interferences with isocratic elution. The method has been successfully applied to the simultaneous determination of matrine and oxymatrine in Sophora Flavescens Ait.. |
| 저자 |
만효룡1, 정용안2, 노경호1
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| 소속 |
1인하대, 2한국기기유화시험(연) |
| 키워드 |
matrine; oxymatrine; Sophora Flavescens Ait.; PR-HPLC
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| E-Mail |
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| 원문파일 |
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