| 학회 |
한국화학공학회 |
| 학술대회 |
2019년 가을 (10/23 ~ 10/25, 대전컨벤션센터) |
| 권호 |
25권 2호, p.2169 |
| 발표분야 |
광감응성 미립자 제조 및 응용기술 심포지엄 (미립자공학부문위원회) |
| 제목 |
Intracellular trafficking of nanoparticles determined by spatiotemporal fluorescence tracking |
| 초록 |
Intracellular distribution of nanoparticles and their interactions with cellular organelles during the course of intracellular trafficking has been emphasized as key information in the field of nano-biotechnology. We investigate intracellular fate of silica nanoparticles at a single nanoparticle level using a complementary imaging analysis which combines transmission electron microscopy and super-resolution confocal laser scanning microscopy with fluorescence correlation spectroscopy. The endocytic pathway internalizes the nanoparticle from the plasma membrane to a set of endosomes over time until degraded by the cellular autolysosomes in the perinuclear region. Spatiotemporally tracing the intracellular behavior of an individual nanoparticle reveals that the nanoparticle is spontaneously released from the endosome and transiently stimulates autophagy. Moreover, the size of autophagosomes increases over time while cell viability is maintained. We show non-cytotoxic biological degradation of the silica nanoparticle, resulting from chemical inertness and stimulated autophagic clearance. |
| 저자 |
주진명 |
| 소속 |
울산과학기술원 |
| 키워드 |
Nanoparticle; silica; cellular trafficking; nano-bio interface
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| E-Mail |
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| 원문파일 |
초록 보기 |
