| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Cellular communication-based screening for export phenotypes |
| 초록 |
Although cellular export is important in industrial biotechnology, its assessment is difficult due to the lack of efficient analytical methods. This study describes a synthetic cellular communication-based microfluidic platform for screening improved export phenotypes. Metabolite-secreting cells were compartmentalized in droplets, with sensing cells equipped with a genetic circuit that converts the export level into an easily detectable signal. This platform was applied to identify Escherichia coli genes that enhance the exportation of 3-hydroxypropionic acid, an industry-relevant platform chemical. Resultantly, two genes (gene X, gene Y) found by this platform enhance the secretion of 3-hydroxypropionic acid and its production. Given the increasing design capability for chemical-detecting cells, this platform has considerable potential in identifying efflux pumps for many important chemicals. |
| 저자 |
김승진1, 진시형2, 임현규1, 이창수2, 정규열1
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| 소속 |
1POSTECH, 2충남대 |
| 키워드 |
Exporter screening; 3-hydroxypropionic acid
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| E-Mail |
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