| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Enzymatic reactive separation of highly water insoluble substrate using bicontinuous interfacially jammed emulsion gels (bijels) as media |
| 초록 |
Although enzymes are efficient catalysts capable of converting various substrates into desired products with high specificity under mild conditions, their effectiveness as catalysts is substantially reduced when substrates are highly water-insoluble. Here, to expedite the enzymatic conversion of a hydrophobic substrate, we used a bicontinuous interfacially jammed emulsion gel (bijel) which provides large interfacial area between two immiscible liquids: oil and water. Using lipase-catalyzed hydrolysis of tributyrin as a model reaction in a batch mode, we show that bijels can be used as media to enable enzymatic reaction. The bijel system gives a four-fold increase in the initial reaction rate in comparison to a stirred biphasic medium. Our results demonstrate that bijels are powerful biphasic reaction media to accelerate enzymatic reactions with various hydrophobic reagents. |
| 저자 |
차상학1, 정규열1, 임현규1, Martin F. Haase2, Kathleen J. Stebe2, 이대연2
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| 소속 |
1POSTECH, 2Univ. of Pennsylvania |
| 키워드 |
enzyme; metabolic engineering
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| E-Mail |
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