| 학회 | 한국화학공학회 |
| 학술대회 | 2003년 봄 (04/25 ~ 04/26, 순천대학교) |
| 권호 | 9권 1호, p.458 |
| 발표분야 | 생물화공 |
| 제목 | Fabrication of DNA/Protein G hetero-self-assembly Layer and its application to immunosensor |
| 초록 | The proteomics-based analytical tool consists of threebasic technologies. One of the most important points in the design of a protein chip is the proper choice of immobilization methode that will retain the activity of protein ligand molecule. In this study, we investigated three cases. First case is self-assembled 11-mercaptoundecanoic acid (MUA) and protein G on gold surface by chemical binding. Second is self-assembled DNA A1 and on gold surface by chemical binding. Third is protein G and complementary DNA layer on mixed self-assembled MUA and DNA A1 on gold surface. This is for the steric effect. And we compared the efficiency of 3 cases as the immunosensor through the antigen-antibody interaction. Bovine serum albumin (BSA) and anti-BSA are used as antigen and antibody. The effect of 3 cases were investigated surface plasmon resonance (SPR) and atomic force microscopy (AFM) and investigated the possibility to the immunosensor. |
| 저자 | 박기숙, 천범석, 이우창, 이원홍, 최정우 |
| 소속 | 서강대 |
| 키워드 | Protein G; DNA; SPR; AFM |
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