| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 봄 (05/02 ~ 05/04, 여수 엑스포 컨벤션) |
| 권호 |
20권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Improvement of polyketide production through the CoA pathway engineering |
| 초록 |
Polyketides are one of the most important and remarkably diverse classes of natural products, which possess a wide range of pharmacological activities. Their carbon backbones are assembled by the successive condensation of short organic-CoA ester extender units through the action of polyketide synthases. In spite of the fast rate of growth, its poor intracellular reservoir of the (2S)-ethylmalonyl-CoA building block that is assembled into polyketides has restained the utility of S. venezuelae as heterologous host. In this report, we firstly identified the ethylmalonyl-CoA biosynthetic pathway. Next, meaA gene encoding coenzyme B12-dependent ethylmalonyl-CoA mutase was deleted and positive regulator gene pikD was introduced into the mutant strain to upregulate tylosin PKS expression. Consequentially, this research demonstrated more than 10-fold increase in the production of tylactone. |
| 저자 |
JIN SHIYING, 신혜령, 최하경, 범지윤, 이지영, 윤여준
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| 소속 |
이화여자대 |
| 키워드 |
polyketides; Streptomyces venezuelae; heterologous host
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| E-Mail |
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