| 학회 |
한국고분자학회 |
| 학술대회 |
2019년 가을 (10/09 ~ 10/11, 제주컨벤션센터) |
| 권호 |
44권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 II |
| 제목 |
Exosome isolation and measurement system using magnetic separation in microfluidics for cancer diagnosis |
| 초록 |
Exosomes have several substances in it that are higly associated with human diseases. However, there is small amounts of disease-derived exosomes exist in the body fluid, so isolating them efficiently is a important challenge. Conventional separation methods using density or size require pre-treatment or complex machine and have low specificity. The problem was overcome by using a different sizes of magnetic nanocluster (MNCs), having a property of varying magnetization according to size. Using the appropriate antibodies, small MNCs were attached to normal exosomes, while large MNCs capture the disease-derived exosomes. Thereafter, the exosomes captured by MNCs were flowed into the microfluidic chip made by lithography, and have different attraction to the magnet by the degree of magnetization, so they are separated and go out to different outlets. In conclusion, our exosome isolation system has high specificity and can have potentially to rapid diagnosis and monitoring of diseases. |
| 저자 |
정혜인1, 문병걸1, 강병훈2, 신무광1, 손혜영1, 노현욱1, 허용민1, 함승주1
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| 소속 |
1연세대, 2생명공학(연) |
| 키워드 |
magnetic separation; exosome; microfluidics; cancer diagnosis
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| E-Mail |
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