| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Quantitative analytical protocol for endogenous estrone in human breast cancer using MALDI-MS |
| 초록 |
Estrone is a major steroid hormone in women and is known to have a high correlation with the incidence of postmenopausal breast cancer. In this study, we develop a highly sensitive quantitative platform based on mass-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to analyze the absolute amount of endogenous estrones in various clinical specimens. Derivatization of endogenous estrone has resulted in excellent linearity (R2＞ 0.99) and improved sensitivity platform (quantification limit: 11 fmol). In addition, the absolute amount of endogenous estrone could be confirmed by using deuterated estrone as an internal standard substance in the cells of the breast cancer cell line MCF-7 (34 fmol / 106 cells). Finally, we have successfully monitored the changes in the level of metabolic expression of estrogen (17.7 fmol / 106 letrozole treated cells) in MCF-7 cells treated with aromatase inhibitors via a MALDI-MS based quantitative platform. |
| 저자 |
황철환1, 박한규1, 송원석2, 안다희1, 정윤이1, 김성민1, 김윤곤1
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| 소속 |
1숭실대, 2서울대 |
| 키워드 |
breast cancer; metabolite; estrone; MCF-7 cell; MALDI-MS;
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| E-Mail |
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