| 초록 |
L-tyrosine is a commercially important compound in the food, pharmaceutical, chemical. Several researches to improve L-tyrosine production have been implemented, but regulation of translation-level expression and carbon flux rebalancing around phosphoenolpyruvate (PEP) nod still has proven to be achieved for optimizing the pathway. Here, we modulated metabolic pathway by fine-tuning gene expression levels to improve L-tyrosine production in Escherichia coli. To optimize the L-tyrosine biosynthetic pathway, a synthetic constitutive promoter and a synthetic 5’-untranslated region were introduced for each gene of interest on the chromosome to allow for control at both transcription and translational levels. Rebalancing of carbon flux was achieved by controlling the expression level of PEP synthetase using UTR Designer. The L-tyrosine productivity of the engineered E. coli strain, SCK5, was increased through pathway optimization resulting in 3.0 g/L of L-tyrosine titer. |
