| 초록 |
Several attempts to improve L-tyrosine production have been implemented, but regulation of translation-level expression and carbon flux rebalancing around phosphoenolpyruvate (PEP) nod still remain to be achieved. Here, we redistributed and optimized the metabolic pathways by precise control of the carbon flux between L-tyrosine biosynthetic pathway and the TCA cycle at the PEP node for maximum L-tyrosine production in Escherichia coli. A synthetic constitutive promoter and 5’-untranslated region (5’-UTR) were designed to control at both transcription and translational levels and carbon flux was rebalanced by controlling the expression level of PEP synthetase using UTR Designer. Using this approach, the L-tyrosine productivity of the engineered E. coli strain was increased resulting in 3.0 g/L of L-tyrosine titer, 0.0354 g L-tyrosine/h/g DCW of productivity, and 0.102 g L-tyrosine/g glucose yield. |
