| 초록 |
We designed multifunctional cell coating materials to improve anticancer efficacy of NK cell. In order to NK cell coating, 2 mg/mL of materials were treated to cells and incubated at R.T. for 15 min. Lipid chains within this platform can penetrate cell membrane and can be anchored without intracellular penetration by PEG. Moreover, NK cell aggregation was inhibited after cell coating. These results demonstrated that when the surface-engineered NK cells are administrated into the body through a syringe, they couldn’t obscure the needle and could facilitate blood vessel escape. In addition, proliferation and function of NK cells were not inhibited after cell coating. The pH responsive S-S bond was dissociated in cancer microenvironment like acidic condition and released antifolate. Furthermore, cancer targeting ability of NK cells was enhanced by folate mediated cancer recognition. Therefore, NK cell coating technique could be used as an anti-cancer therapy with enhanced efficacy. |
