| 초록 |
In this study, we used pH-sensitive fluorescent carbon dots (FCD) with excellent photoluminescence properties and high biocompatibility. Using pH-sensitive phenyl boronic acid, FCD was quenched by binding with the fluorescent probe (DYE) to form FRET quenching. FCD-DYE bonded by a boronate ester bond can preferentially bind to a diol group of the bacterial cell surface due to its preferential affinity for a diol-containing molecule. In bacterial conditions, DYE is released from the FCD, and FCD produces a cyclic ester bond with the bacterial diol group, causing fluorescence to be recovery. This system can be applied as a biosensor whose strength is its simplicity and high sensitivity to bacteria detection. This work supported by Korea Institute for Advancement of Technology(KIAT) grant funded by the Korea Government(MOTIE) (P00008500, The Competency Development Program for Industry Specialist). |
