| 초록 |
Exosomes of human mesenchymal stem cells (hMSC) are known to help cartilage regeneration. However, the effect of exosome of chondrocyte to hMSC has not been investigated. Therefore, the present study was designed to investigate exosome-mediated bidirectional signalings between hMSC and chondrocytes with various culture media formulations. The exosomes isolated from bovine chondrocytes (BC) and hMSC (50 μg/ mL of exosomes per 3000 cells) were treated to hMSC and BC, respectively. Both cells were cultured in media formulations with 10 % FBS, 10 % exosome free-FBS, and 0.5% FBS. The cellular responses of hMSC and BC including proliferation, chondrogenic differentiation, cartilage ECM deposition were evaluated. As a result, exosome treatments increased proliferation of both BC and hMSC, and similar bidirectional influences were also upregulated in both cell populations. Moreover, exosome-mediated in vitro activation between two cell populations could be regulated by media formulations. |
