| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Investigation of metabolic reprograming in CK2α-overexpressed colon cancer cells by carbon isotope using GC/MS |
| 초록 |
CK2α is a serine/threonine kinase that is constitutively active in multiple cancer cells. Using colon cancer cell lines such as HT29 and SW620, CK2α induced epithelial mesenchymal transition and the transitioned cells became more proliferative. We assumed that CK2α could affect cancer cell growth by modulating energy metabolism. Therefore, we examined the reprogrammed metabolic fluxes of CK2α on glucose metabolism via isotope labeled substrates and could successfully determine the relative amount and mass isotopomer distribution(MID) of metabolites using GC/MS. The results showed that continuous activation of CK2α facilitates the Warburg effect and anaplerotic pathway. Besides, results also showed CK2α overexpression affected glutamine uptake metabolism, varying amount and MID of TCA intermediates. This study suggests that because oncogenes regulate important metabolic enzymes and metabolism signaling pathways, CK2α is an excellent metabolic target for cancer therapy. |
| 저자 |
임대균1, 양경미2, 정희선3, 이종석4, 오민규1
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| 소속 |
1고려대, 2연세대, 3국립암센터, 4경기도경제과학진흥원 |
| 키워드 |
GC/MS; colon cancer; carbon labeling experiment; mass isotopomer distribution
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| E-Mail |
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