초록 |
Biological production of medium chain carboxylic acids (MCCAs) by Caproiciproducens galactitolivorans during anaerobic fermentation using biodiesel-waste glycerol as a carbon source was investigated. Crude glycerol is a low-value by-product of the biodiesel production process and a convenient feedstock alternative for the carboxylate platform. In this study, a well-known MCCAs producing bacterium, Caproiciproducens galactitolivorans produced caproic acid as a major metabolic product of anaerobic fermentation with crude glycerol. To further enhance the production of caproic acid, also addition of a non-ionic surfactant, Tween 80, was performed. As a result, 10 g/L of caproic acid production was obtained with 2% Tween 80 supplementation. Moreover, through the in-situ biphasic extractive fermentation, the highest production and production rate were achieved. |