| 초록 |
In this study, the effect of fluorination to arginine side chains of linear bioreducible polymers were investigated. Heptafluorobutyrated arginine functionalized poly(cystamine bisacrylamide-diaminohexane)s (FR-PCHs) were synthesized. They could form positively charged and nano-sized polyplexes with pDNA. Interestingly, highly fluorinated FR-PCHs could condense pDNA even in reducing conditions, probably due to the hydrophobic interaction of fluoroalkyl chains. FR-PCHs showed comparable transfection efficiency to PEI25k in serum-free conditions and superior efficiency to PEI25k even in serum-containing conditions, indicating the good serum-compatibility. Fluorination could increase the cellular uptake with high membrane permeability of FR-PCHs. Through cellular uptake inhibition analysis, it was suggested that endocytosis of FR-PCH polyplexes would be mediated by multiple pathways. |
