| 학회 | 한국화학공학회 |
| 학술대회 | 2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터) |
| 권호 | 16권 2호, p.1497 |
| 발표분야 | 분리기술 |
| 제목 | Purification of immunoglobulin IgY from chicken egg yolk |
| 초록 | Recently chicken egg yolk has been considered as an ideal alternative antibody source. The production for antibody in eggs and the extraction of spectific antibody from egg yolk are increasingly attracting the interest of the sxientific community. Although some of the separated egg yolk components which are water, lipoprotein and protein are already commercialized, the separation efforts are limited to a pure component. IgY has Particularly a equivalent function withIgG which is in serum. This studywas carried out to separate and analyze IgY from chicken egg. To perform this experiment, egg yolk powder diluted with cold water was centrifuged after adjusting the pH to 5.0. 0.01% carrageenan wasadded to reduce the lipid. The supernatant was filtered through a Whatman no. 1 filter paper. The concentrated sample was loadedonto a column equilibrated with 20 mM phosphate buffer at pH 7.0 and eluted with phosphate buffer and 1M NaCl. IgY was analytically separated by using ion exchange, gel filtration and high pressure liquid chromatography. By changing the amount of egg yolk powder, eluted height of peaks were quantitatively changed. |
| 저자 | 송성문, 김인호 |
| 소속 | 충남대 |
| 키워드 | IgY; Egg yolk; DEAE |
| 원문파일 | 초록 보기 |
