| 초록 |
An integrated centrifugal microfluidic device based on colorimetric detection of various pathogens was modified and characterized for colorimetric detection of various microbial pathogens. Microbeads-assisted extraction and purification of bacterial genomic DNA, target DNA amplification and detection were fully integrated. A highly efficient detection method of colour change during target DNA amplification was developed and optimized. The detection of pathogens was demonstrated using this integrated centrifugal microfluidic device. Acknowledgement: This research was supported by the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI13C1232). |
