| 학회 |
한국고분자학회 |
| 학술대회 |
2015년 가을 (10/06 ~ 10/08, 대구컨벤션센터(EXCO)) |
| 권호 |
40권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 II |
| 제목 |
Nanoparticle Based on L-Tyrosine Polyurethanes for DNA Delivery |
| 초록 |
A biodegradable polyurethane has been developed using L-tyrosine (LTU2a). LTU2a was designed to degrade through hydrolysis and enzymatic mechanisms since the backbone of the polymer contains a peptide bond. Using water-in-oil-in-water emulsion, nanoparticles encapsulating complexes of DNA and linear polyethylenimine LPEI or bovine serum albumin labeled with fluorescein have been fabricated. In addition, their surfaces have been converted with PEG. The morphology is spherical. After 14 days, 15 ng of DNA per mg of nanoparticles have been released from the LTU2a nanoparticles. Furthermore, a successful uptake of LTU2a nanoparticles by the hepatic cells has been confirmed with confocal laser microscope. These characteristics are ideal for drug-delivery systems designed to transport and release drugs in the cytoplasm. Therefore, LTU2a nanoparticles could be a promising for gene-delivery applications that require sustained release of DNA for the duration of a cell’s lifetime. |
| 저자 |
Madhusudana Rao1, 윤양현2, 정일두1, 박수용1
|
| 소속 |
1부산대, 2The Univ. of Akron |
| 키워드 |
DNA delivery; nanoparticle; biodegradable; water-in-oil-in-water emulsion
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| E-Mail |
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