| 학회 | 한국화학공학회 |
| 학술대회 | 2015년 가을 (10/21 ~ 10/23, 일산 KINTEX) |
| 권호 | 21권 2호, p.1778 |
| 발표분야 | 생물화공 |
| 제목 | Expression of Recombinant Cellobiose Dehydrogenase from Phanerochate chrysosporium and its Application to Biofuel Cell |
| 초록 | The cellobiose dehydrogenase (CDH) is considered as capable element of carbohydrates detector in biofuel cell application based on its versatile biorecognition. Recently, CDH has been studied as an important candidate for anode biocatalyst for enzymatic fuel cells based on its positive performance of electron transmitter. In this study, a gene encoding cdh from Phanerochate chrysosporium has been cloned into pPICZαB and expressed in the methylotrophic yeast Pichia stipitis, under the control of the AOX1 methanol inducible promoter. The recombinant CDH (rPcCDH) was purified using a metal ion affinity chromatography column under non-denaturing conditions for further application on modified electrode. The biofuel cell was established with purified rPcCDH and laccase on anode and cathode, respectively. The performance of this biofuel cell evaluated that cell’s open circuit voltage was 0.435 V and the maximum power density was 310 ± 5.2 μW/cm2 at 0.247 V in an air-saturated sodium acetate buffer with pH 4.5 at 37 °C containing 10 mM lactose. |
| 저자 | 김승욱, 이수권, 이주훈, 이자현, 김동섭, 최한석 |
| 소속 | 고려대 |
| 키워드 | Cellobiose dehydrogenase; Phanerochate chrysosporium; Biofuel cell |
| 원문파일 | 초록 보기 |
