| 초록 |
Elementary modes (EMs) generated from the central carbon metabolic network of M. succiniciproducens were clustered to systematically analyze SA production routes. Based on the results of EMC analysis, zwf gene was identified as a novel overexpression target for the improved SA production. This gene was overexpressed in SA-overproducing M. succiniciproducens LPK7 strain. Heterologous NADPH-dependent mdh was selected for overexpression to synergistically improve SA production by utilizing abundant NADPH pool mediated by the overexpressed zwf. The LPK7 strains co-expressing mdh alone and both zwf and mdh genes were subjected to fed-batch fermentation to examine their SA production performances. Strategies of EMC analysis will be useful for further metabolic engineering of M. succiniciproducens and other microorganisms to improve production of SA and other chemicals of interest. [This work was supported by the C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2016M3D3A1A01913250)] |
