| 학회 | 한국공업화학회 |
| 학술대회 | 2017년 가을 (11/08 ~ 11/10, 부산 벡스코(BEXCO)) |
| 권호 | 21권 2호 |
| 발표분야 | 환경에너지_포스터 |
| 제목 | Evaluation of different alcohol dehydrogenases for bio-butanol production |
| 초록 | Bio-butanol is an attractive chemical that can be used as an alternative oil-based transport fuel and precursor of various chemical compounds. In general, bio-butanol could be produced by the reversed β-oxidation pathway constructing 4-5 genes. Among genes related to the pathway, alcohol dehydrogenase (adh) known for terminal enzymes is a significant enzyme. In order to evaluate adh in-vivo, butanol producing E. coli platform containing the acyl-CoA transferase gene (act, converting carboxylic acid into acyl-CoA) derived from Megasphaera hexanoica MHT was constructed. After that, four adhs (adhE, adhE2 and PduP-AdhA) were inserted and expressed in the strain, respectively. Productivities of four adhs were compared for performance evaluation. As a result, ranks for productivity showed like follows, PduP-AdhA＞adhE＞adhE2. (1.7 g/L/h, 1.1 g/L/h, 0.8 g/L/h, respectively) Finally, it is expected that these studies can affect increase of butanol production based on biological process. |
| 저자 | 신승진, 전병승, 상병인 |
| 소속 | 한양대 |
| 키워드 | Bio-butanol; acyl-CoA transferase; alcohol dehydrogenase |
