| 초록 |
The goal of this work is to develop “smart materials for tissue engineering and biosensors” in combination with Micro-Electro-Mechanical Systems (MEMS) technology. In the first part of the work, a method for capture, analysis, and sorting of live cells using a photodegradable hydrogel (photogel) functionalized with antibodies will be demonstrated. Photogel substrates were used to isolate human CD4 or CD8 T-cells from a heterogeneous cell suspension and then to release desired cells or groups of cells by UV-induced photodegradation. As the second part of the work, biomarkers related with diseases including proteases were detected by using hydrogel based biosensors. This method will be providing numerous real time secretion profiles rather than conventional detection methods including ELISA, PCR and gel electrophoresis. |
