| 초록 |
A novel class of ternary complex was prepared via electrostatic interaction between negatively charged dendrimer phthalocyanine and positively charged polyplex. Control of the transgene expression in the body is a main issue in the gene therapy. However, the existing vectors including viral and non-viral ones might lack the ability of controlling the transgene expression. Use of external stimuli for enhancement of the transgene expression may be a promising approach to the site-directed transfection in vivo. Here, we have constructed a novel supramolecular complex composed of the core containing the packaged DNA and the envelope of anionic dendrimer phthalocyanine, which resembles a viral architecture. The virus-mimicking complex showed more than 100-fold enhancement of transgene expression in a light-selective manner without the cell mortality, and therefore, achieved the light-directed in vivo gene transfer to conjunctival tissues in rats. Our results clearly suggest a success of the site-directed, in vivo gene transfer using non-viral gene vectors, giving future promise of non-viral gene therapy. |
