| 초록 |
We illustrate the design and development of a safe, efficient and nonreplicable rHbsAg oral delivery system using biocompatible and biodegradable nanocarrier for eliciting cellular as well as humoral immune responses against hepatitis B virus. An as-designed antigen-loaded nanocarrier can enhance both mucosal and cellular immunity at a time because our selected polysaccharide either itself or in conjugation with M-cell homing peptide can target follicle-associated epithelium region of Peyer’s patch whereas positive charge polymer has the ability to stabilize nanocomplex with antigen and localize into the nucleus. Initially, we used BSA as a reference protein instead of rHbsAg to investigate various characterization properties and in vitro studies of our proposed nanocarrier. To date, various test results of our current study indicate that our developed vaccine carrier would be a reliant alternative to the needle-based hepatitis B vaccine. |
