| 초록 |
In this study, we confirmed the increase in PHB production in recombinant Escherichia coli due to overexpression of the response regulator dr1558 gene in MR medium containing wood hydrolyzate. Recombinant E. coli in the medium containing wood hydrolyzate had low cell concentration and PHB content, but the cell concentration increased by 10% or more and PHB concentration by 70% or more by expression of dr1558. As a result of transcriptome analysis, it was confirmed that overexpression of dr1558 increased the expression of TCA cycle-related genes and PHB biosynthetic genes. The results of this study are thought to be useful for the fermentation production technology of biodegradable polymer PHB and other biological materials using low-cost wood hydrolysates. In addition, the dr1558-expressing recombinant E. coli produced in this study is thought to be applicable to various fields such as the production of organic acids and the production of GABA using cadA having activity at low pH, and it is considered that it can be industrialized. |
