| 초록 |
Hyaluronic acid (HA) is a natural linear polysaccharide composed of alternating disaccharide units of D-glucuronic acid and N-acetyl-D-glucosamine. Because of its excellent physico-chemical properties such as biodegradability, biocompatibility, non-toxicity and non-immunogenicity, HA has been used for various medical applications. The objective of this study was to develop a bioconjugate system for a peptide drug using HA as a novel drug carrier. The peptide drug used in this study (WRYMVm) was one of the agonistic peptides for formyl peptide receptor-like 1 (FPRL1) receptor. To introduce a thiol group, cysteine was added to the peptide sequence, which resulted in CWRYMVm. HA-peptide conjugate formed via Michael addition reaction was fractionated using GPC column. 1H NMR analysis confirmed the formation of HA-peptide conjugate. Cell activity test confirmed the biological activity of HA-peptide conjugate elevating the level of extracellular signal mediated kinase (ERK) in the cell. |
