| 학회 |
한국화학공학회 |
| 학술대회 |
2020년 가을 (10/14 ~ 10/16, e-컨퍼런스) |
| 권호 |
26권 1호, p.299 |
| 발표분야 |
생물화공 |
| 제목 |
Evolutionary metabolic engineering of the 3-hydroxypropionic acid biosynthetic pathway using a synthetic biosensor |
| 초록 |
Evolutionary metabolic engineering has great progress for improving the metabolite-producing strains. In this case, it is important to develop an efficient screening method because of the low probability of incidence of positive mutants in a library. Therefore, in this study, a synthetic biosensor using a transcription regulator was constructed and optimized for the efficient accomplishment of the evolutionary metabolic engineering. As the target metabolic pathway, the production pathway of 3-hydroxypropionic acid (3-HP), one of the important platform chemicals, has been adopted. The results indicate the successful applications to the 3-HP production pathway engineering, especially for enzyme engineering. Therefore, the strategy used in this study could expedite the strain improvement in the field of metabolic engineering by developing a high-throughput screening method. |
| 저자 |
석주연1, 양진아2, 임현규1, 한용희2, 김성경1, 최상진3, 유태현3, 서상우2, 정규열1
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| 소속 |
1포항공과대, 2서울대, 3아주대 |
| 키워드 |
바이오화학 |
| E-Mail |
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| 원문파일 |
초록 보기 |
