| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 가을 (10/06 ~ 10/07, 김대중 컨벤션센터) |
| 권호 |
36권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
DNA-Funtionalized Nanochannels for SNP Detection |
| 초록 |
In this study, we introduced a new concept of DNA-based molecular recognition agents (MRAs) in detecting the single nucleotide polymorphisms (SNPs) with very high precision and efficiency. For this purpose, we fabricated ultrahigh-density array of functional nanochannels having walls covered with carboxylic acid, which served as highly sophisticated molecular recognition probes by using a block copolymer. This approach provides a facile route for direct functionalization of wall surface of the nanochannels and immobilization site for MRAs. Finally, using overhanging single-strand DNA as MRAs, the DNA-functionalized nanochannels showed high resolution to detect a single-base mismatch as well as to discriminate single-mismatched sequence at various locations by hybridization preference with MRAs. |
| 저자 |
손세진, 양승윤, 김현우, 장상신, 전금혜, 김진곤, 김원종
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| 소속 |
포항공과대 |
| 키워드 |
single nucleotide polymorphisms (SNPs); nanochannel
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| E-Mail |
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