| 초록 |
Cartilage lesions are relatively common but remain obstacles for clinicians and researchers due to their inadequate self-healing capacity. Recently, an ideal decellularized extracellular matrix (dECM) based scaffold has been developed to regenerate cartilage damage. In regenerative medicine, dECM approaches have been rapidly expanding due to their instructive cues for cell attachment, proliferation, differentiation, and most importantly, it provides a tissue native environment. The objective of the present study was to develop a 3D printable hydrogel-based bio-ink by mixing dECM with TEMPO-Oxidized cellulose nanofiber (TOCN) because of its unique properties, including sustainability, good mechanical properties, biodegradability, and high surface area. In addition, Sodium alginate (SA) was added together with the dECM-TOCN gel because of its easy and fast cross-linking ability. Three formulations of bio-inks were prepared with different proportions of dECM/TOCN: 80/20, 50/50, 20/80. Bio-inks were partially cross-linked before printing with calcium chloride solution (3% based on dry alginate) to enhance printability. After printing, the 3D bio-printed constructs were finally cross-linked immediately by 100mM calcium chloride solution to impart rigidity and long-term stability. For cartilage regeneration, the newly developed printed constructs from bio-inks were subjected to evaluation for printability and in-vitro cell biocompatibility. It was observed that 3D bio-printed dECM-TOCN scaffolds provided a favorable microenvironment for cell adhesion, proliferation, promoted chondrogenesis, and thus could be a promising strategy for cartilage regeneration. |
